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Edit protocol

Design of Blueprint

Software used to draw 3D module

Name of software

Dimensions of 3D model

Units: mm

Software used to convert 3D model to bioprinting protocol and toothpath

Name of software

Spacing between lines

Units: mm

Porosity of model

Units: %

Layer thickness

Units: µm

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Upload file

File format: Gcode

File name:

File size:

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Bioinks

Biomaterial used

	Biomaterial	Company supplying biomaterial
1
1

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Composition

Formulation of bioink

Brief description of synthesis or production process

References

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Cells

Cell type(s)

Culture media composition

Cell concentration

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Bioprinter

Model and Manufacturer

Developed In-house

Yes

Brief description and reference (if possible)

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Bioprinting technology

Describe, references

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Bioprinting Parameters

Inkjet - Piezoelectric or Thermal

Inkjet – Piezoelectric or Thermal

Pneumatic-driven microextrusion

Piston-driven microextrusion

Screw-driven microextrusion

Pressure

Units: kPa, Pa, Bar, PSI)

Flow rate

Units: µL/min, µL/s

Printing speed

Units: mm/min, mm/s

Valve opening tme

Units: µs

Screw velocity

Units: rpm

Dosing distance

Units: mm

Printhead temperature

Units: °C

Printbed temperature

Units: °C

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Crosslinking process of bioink

Describe briefly

Crosslinking method

Wavelength

Units: nm

Concentration

Temperature

Describe

Crosslinking time

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Bioprinting metrics

Time for bioprinting and crosslinking process per construct

Volume of bioink per construct

Units: µL, mL

Dimensions of bioprinted construct

Units: µm, mm

Line resolution

Units: µm, mm

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Post-bioprinting

Post bioprinting

Time of 3D culture in vitro

Stability of constructs in vitro?

Macroscopic evaluation of constructs

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Results

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Rheological characterization of bioink

Printing resolution of bioink

Cell viability

Cell morphology

Cell proliferation

Neo-synthesis of extracellular matrix

Other

Biomechanical analysis

Biochemistry analysis

Gene expression

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