Protocols

Silicone Nose

Protocol

Design of Blueprint

Software used to draw 3D model

Dimensions of 3D model

20x12x12

Software used to convert 3D model to bioprinting protocol and toolpath

Name of software

Slic3r + INKREDIBLE POSTPROCESSOR

Porosity of model

50

Layer thickness

300

Bioinks

Biomaterial used

PDMS (SE 1700)

Company supplying biomaterial

Dow Corning

Composition

Formulation of bioink

10:1 Base to curing Agent

Cells

Cell type(s)

no cells

Cell concentration

no cells

Culture media composition

no cells

Bioprinter

Model and Manufacturer

INKREDIBLE by CELLINK

Bioprinting Technology & parameters

Pneumatic-driven microextrusion

Pressure

185 KPa

Printing speed

4800 mm/min

Printhead temperature

RT

Printbed temperature

RT

Crosslinking process of bioink

Heat-curing

Crosslinking method

Thermal

Temperature

room temperature

Crosslinking time

48hrs

Bioprinting metrics

Time for bioprinting and crosslinking process per construct

3 mins printing

Post-bioprinting

Results

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