Protocols

Sheep Meniscus

Protocol

Design of Blueprint

Software used to draw 3D model

Name of software

A 3D model of a sheep meniscus was reconstructed from a CT scan. The STL file of the meniscus model was processed in the software Slic3r (v1.2.9). Organ Support was selected as the print setting. This profile prints the perimeter and infill of a 3D models with printhead 1 (PH1) and the support material is printed with PH2, where the support material contains no cells.

Software used to convert 3D model to bioprinting protocol and toolpath

Spacing between lines

Layer Height = 0.40mm

Porosity of model

External perimeters extrusion width = 0.40mm

Layer thickness

Perimeters = 1

Bioinks

Biomaterial used

Bioink for bioprinting of perimeter and infill: CELLINK Start
Bioink of bioprinting the supporting structures: CELLINK Support

Company supplying biomaterial

CELLINK
CELLINK

Composition

Cells

Cell type(s)

No cells used

Cell concentration

N/A

Culture media composition

N/A

Bioprinter

Model and Manufacturer

INKRIBLE 3D Bioprinter by CELLINK

Bioprinting Technology & parameters

Pneumatic-driven microextrusion

Pressure

Printing pressure for PH1: 100-110 kPa
Printing pressure for PH2: 50-60 kPa

Printing speed

PH1 and PH2: 600 mm/min

Printhead temperature

Room temperature (22°C)

Printbed temperature

Room temperature (22°C)

Crosslinking process of bioink

Not applicable. CELLINK Start and CELLINK Support are non-crosslinkable, sacrificial materials – perfect for getting started with 3D bioprinting. You can print all your complex structures with CELLINK Start and ensure that they look the way they should prior to printing with human cells. It’s the perfect start for all your bioprinting experiments.

Crosslinking method

Other

Bioprinting metrics

Time for bioprinting and crosslinking process per construct

13 minutes per construct

Post-bioprinting

Time of 3D culture in vitro

N/A

Stability of constructs in vitro?

N/A

Results

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