Protocols

Printing dual droplets (PH1 & PH2)

Protocol

Design of Blueprint

Software used to draw 3D model

Software used to convert 3D model to bioprinting protocol and toolpath

Spacing between lines

4 mm

Bioinks

Biomaterial used

CELLINK
GelMA A

Company supplying biomaterial

CELLINK
CELLINK

Composition

Cells

Cell concentration

10% cell suspension

Bioprinter

Model and Manufacturer

CELLINK BIO X

Description

A G-code for BIO X for printing one droplet with PH1 and then another droplet with PH2 4 mm beside the first droplet.

Bioprinting Technology & parameters

Pneumatic-driven microextrusion

Pressure

CELLINK Bioink was printed at 15-20 kPa, while GelMA A required higher pressure of 35-40 kPa.

Crosslinking process of bioink

Crosslinking method

UV

Bioprinting metrics

Post-bioprinting

Results

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