Hi everybody! I've been working with the BioX for months only using the pneumatic printheads; now I'm trying the syringe pump printhead because I'm looking for a more controlled extrusion (and to use it with the FRESH method). My questions are: 1) Does anybody already used it and with wich parameters of flow rate? (I'm going to use Cellink Bioink and GelXA) 2) I'm in trouble with the syringe because when the print process is over (so no mechanical pressure is applied to the syringe) the bioink continues to go out from the nozzle. Thank you very much.
1) Here is a G-code to control the syringe head: G21 ; set units to millimeters G90 ; use RELATIVE coordinates M83 ; T0 G1 Z15 ;H1 G1 X0 Y0; G1 Z0.2 ; M400 ;Begin Row 1 M2045 T0 R M2032 T0 S10000; nL/sec, off by a factor of 1.48, for example 20 sec theortical takes 13.75 seconds. M2051 T0 V200000; 200 ul 200000 nL G4 S2 G1 Z15 ;Restore Safe Z Height You can set the volume dispended with M2501 Vx where the volume (x) is in nL The extrusion rate is set through M2032 Sx where S is in nL/sec.’ Try a speed of 15 mm/s, Extrusion rate of 10mm/s and a retraction volume of 20 ul with a 22G needle. Which software version are they using as version 1.7.1 seems to be working. 2)There are 2 parameters including Extrusion rate and retract volume. Please use the retraction volume feature when setting up the printing protocol. Start with 50uL as the retraction volume. Try having retract volume the same as extrusion rate. Should stop extrusion once the print is done.
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