Mix all the component with a lot of energy due de high viscosity and then put in and falcon tube with centrifuged at 7000 rpm 10 min to take off all the bubles, then take the gel and put it inside one syringe for bioprinter and print with a pressure over 400 Kpa
Nose Lattice is a work with Grashooper Rhinoceros developed to print with different pore size, bean thickness and topology to created trabecular structures and test it with a cells culture . casilvaca@unal.edu.co
No crosslinking
It must to be keeped in PBS or DMEM F12 Media because after one day it star to become dry and the structure changes
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Nozzle 400 Microns , 100 microns Z layer
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