After the bioprinting process, the cell-laden ear constructs were crosslinked by submerging in an ionic solution of 100mM CaCl2 for 10 minutes. The constructs were then rinsed and incubated in culture medium in standard culture conditions (37°C, 5% CO2 and 95% relative humidity).
Macroscopic evaluation of constructs showed no change in shape during in vitro 3D culture.
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