Protocols

Human Aortic Valve

Protocol

Design of Blueprint

Software used to draw 3D model

Name of software

Human aortic valve was segmented and reconstructed from CT images using 3D Slicer software.

Software used to convert 3D model to bioprinting protocol and toolpath

Name of software

Slic3r (v1.2.9)

Spacing between lines

Layer Height = 0.40mm

Porosity of model

External perimeters extrusion width = 0.40mm

Layer thickness

Perimeters = 1

Bioinks

Biomaterial used

CELLINK Start

Company supplying biomaterial

Cellink

Composition

Cells

Cell type(s)

No cells used

Cell concentration

N/A

Culture media composition

N/A

Bioprinter

Model and Manufacturer

INKRIBLE 3D Bioprinter by CELLINK

Bioprinting Technology & parameters

Pneumatic-driven microextrusion

Pressure

Printing pressure for PH1: 100-110 kPa (Nozzle: Straight tip, 22 GA)
Printing pressure for PH1: 50-60 kPa (Nozzle: Conical tip, 27 GA)
Printing pressure for PH1: 30-35 kPa (Nozzle: Conical tip, 25 GA)
Printing pressure for PH1: 25 kPa (Nozzle: Conical tip, 22 GA)

Printing speed

600 mm/min

Printhead temperature

Room temperature (22°C)

Printbed temperature

Room temperature (22°C)

Crosslinking process of bioink

Not applicable. CELLINK Start is a non-crosslinkable, sacrificial material – perfect for getting started with 3D bioprinting. You can print all your complex structures with CELLINK Start and ensure that they look the way they should prior to printing with human cells. It’s the perfect start for all your bioprinting experiments.

Crosslinking method

Other

Bioprinting metrics

Time for bioprinting and crosslinking process per construct

8 minutes and 18 seconds per construct

Post-bioprinting

Time of 3D culture in vitro

N/A

Stability of constructs in vitro?

N/A

Results

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