After the bioprinting process, the cell-laden constructs were crosslinked by submerging in an ionic solution of 100mM CaCl2 for 6 minutes. The constructs were then rinsed and incubated in culture medium in standard culture conditions (37°C, 5% CO2 and 95% relative humidity).
Macroscopic evaluation of constructs showed no change in shape after 7 days of in vitro culture.
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