Nanocellulose and sodium alginate-based bioink
After the bioprinting process, cover the cell-laden constructs with crosslinking solution (100mM CaCl2) and wait for 5 minutes. Remove the crosslinking solution and then rinse the constructs with culture medium. Place the constructs in multiple wellplates (eg. 24 wellplates) prefilled with culture medium (eg. 1mL per well) and incubate in standard culture conditions (37°C, 5% CO2 and 95% relative humidity). Change the media every second day or as often as needed.
During the in vitro 3D culture, you can analyze cell viability, morphology proliferation as well as production of ECM components and gene expression.
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