Composition: CELLINK GelMa is a gelatin-based bioink that provides mammalian cells with a milieu that resembles some essential properties of their native environment. GelMA is modified with methacryloyl substitution groups, which crosslink in the presence of a photoinitiator (eg. Irgacure 2959) and exposure to UV light (eg. 365nm wavelength), yielding a stable gel.
During the bioprinting process, the 365nm UV curing system will crosslink each layer, following the same printing path. The default speed is 600 mm/min. If needed, the speed can be decreased by turning the control knob counter-clockwise in order to increase the crosslinking time for each layer.
Once the bioprinting process is completed, transfer the constructs to multiple wellplates (eg. 24 wellplates) prefilled with culture medium (eg. 1mL per well) and incubate in standard culture conditions (37°C, 5% CO2 and 95% relative humidity). Change the media every second day or as often as needed.
During the in vitro 3D culture, you can analyze cell viability, morphology proliferation as well as production of ECM components and gene expression.
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