Human Organs

NOSE LATTICCE

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Description

Nose Lattice with Grasshooper Rhinoceros

Protocol

Design of Blueprint

Software used to draw 3D model

Name of software

Grasshooper Rhinoceros

Dimensions of 3D model

50 x 80 x 40 But it can be scaled to get a smaller model 35 x 25 x 20 sould be a good zise to print it

Software used to convert 3D model to bioprinting protocol and toolpath

Name of software

Repetier host

Spacing between lines

0.2

Porosity of model

10

Layer thickness

400

Bioinks

Biomaterial used

Methyl cellulose viscosity: 4,000 cP | Sigma-Aldrich

Company supplying biomaterial

Sigma-Aldrich

Composition

Formulation of bioink

Methyl cellulose viscosity: 4,000 cP | Sigma-Aldrich (9%) + Gelatin from porcine skin | Sigma-Aldrich (5%) + dH20 (deionised water) (86%) w/w

References

casilvaca@unal.edu.co

Brief description of synthesis or production process

Mix all the component with a lot of energy due de high viscosity and then put in and falcon tube with centrifuged at 7000 rpm 10 min to take off all the bubles, then take the gel and put it inside one syringe for bioprinter and print with a pressure over 400 Kpa

Cells

Cell type(s)

No cells added yet

Cell concentration

No cells added yet

Culture media composition

No cells added yet

Bioprinter

Model and Manufacturer

Nose Lattice

Description

Nose Lattice is a work with Grashooper Rhinoceros developed to print with different pore size, bean thickness and topology to created trabecular structures and test it with a cells culture . casilvaca@unal.edu.co

Bioprinting Technology & parameters

Piston-driven microoextrusion

Flow rate

Range (0.4 µL/s- 1µL/s)

Printing speed

10

Printhead temperature

Room temperature 15 C°

Printbed temperature

Room temperature 15 C°

Crosslinking process of bioink

No crosslinking

Crosslinking method

Other

Description

No crosslinking

Crosslinking time

No crosslinking

Bioprinting metrics

Time for bioprinting and crosslinking process per construct

136 minutes

Volume of bioink per construct

4. 4 ml

Dimensions of bioprinted construct

(50 x 80 x40) mm

Line resolution

400 µm

Post-bioprinting

Time of 3D culture in vitro

No cell culture yet

Stability of constructs in vitro?

It must to be keeped in PBS or DMEM F12 Media because after one day it star to become dry and the structure changes

Macroscopic evaluation of constructs

No yet

Results

Rheological characterization of bioink

No yet

Printing resolution of bioink

Nozzle 400 Microns , 100 microns Z layer

Cell viability

No yet

Cell morphology

No yet

Cell proliferation

No yet

Neo-synthesis of extracellular matrix

No yet

Biomechanical analysis

No Yet

Biochemistry analysis

No Yet

Gene expression

No Yet

Notes

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